Performance of nanopolyaniline-fungal enzyme based biosensor for water pollution

The Laccases are oxidoreductases belonging to the multinuclear copper-containing oxidases; they catalyse the monoelectronic oxidation of substrates at the expense of molecular oxygen. These essentially ecofriendly enzymes work with air and produce water as the only by-product. Their uses span from the textile to the pulp and paper industries, and from food applications to bioremediation processes. Laccases also have uses in organic synthesis, where their typical substrates are phenols and amines, and the reaction products are dimers and oligomers derived from the coupling of reactive radical intermediates. Laccase from Pleurotus ostreatus was extracted from the Shaken flask cultures of Pleurotus ostreatus and grown at 25°C with continuous agitation (110 rpm) in baffled Erlenmeyer flasks (1000 mL) containing 200 mL medium. The basal glucose yeast extract peptone agar medium (GYP medium) used for cultures unless otherwise stated contained 20 g glucose L -1 , 5 g yeast extract L -1 , 5 g