Uptake and toxicity of different nanoparticles towards a tough bacterium: Deinococcus radiodurans

Nanomaterials (NMs) have found extensive commercial use in industries, healthcare and household applications however, their ecotoxicological effects remain elusive. Since, microbial communities play beneficial role in ecosystem like element cycling, bioremediation, nitrogen fixation, etc., effect of NMs over beneficial microbe’s physiology and viability remains to be studied in detail. Some beneficial microbe communities are severely affected by the release of NMs in the environment. Deinococcus radiodurans is known for its tolerance to oxidative stress caused due to irradiation. In this study, we have used metal, metal oxides, quantum dots (QDs) and carbon based NMs to assess their effect on the cell viability, uptake and ROS generation in D. radiodurans cells. The present study demonstrates in real-time by flow cytometry the internalization of different metal, metal oxide, QDs and carbon based NMs in D. radiodurans. Results show that all the tested NMs are significantly internalized