Synthesis, Characterization and Application of MCM-41@LDH as a New Support for Lysozyme: Central Composite Design to Evaluate Experimental Variables

A precipitation method was used to synthesize MCM-41. Then, the obtained mesoporous was modified using layered double hydroxide (MCM-41@LDH). The novel mesoporous MCM-41@LDH was successfully applied for adsorption of lysozyme (LYS) at different conditions such as adsorbent amount, pH of solution, stirrer time, and concentration of protein which were designed using central composite design (CCD). Furthermore, the chief characteristics of new adsorbent were identified using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDX) analyses. The maximum adsorption of LYS, predicted by CCD was 0.025 g of support, LYS concentration of 300 mg L^-1, pH 7.50, and stirrer time of 55 min. The isotherm, kinetic, and thermodynamic equations of LYS on MCM-41@LDH were surveyed. It was established that Freundlich isotherm (R²=0.997) and second-order kinetic (R²=0.997) were the be